Purpose of the RPPASPACE R Package | An Example to Show How It Works | Expected Results from the Sample Code and Data | The Biology of RPPAs | The RPPASPACE Model | Differences between RPPASPACE and Its Predecessor, SuperCurve | RPPASPACE Input Data | RPPASPACE Standard Slide Input Format | RPPASPACE Expectations of Input Data | Positive and Negative Controls | Slide Layout Example | Handling of Replicated Sample Dilution Series | Support of Missing or Skipped Sample Series | Randomly Ordered Slides | The RPPASPACE Process | Read Slide Quantification Files | Perform Pre-Fit Quality Assurance Check | Perform Spatial Adjustments | Perform Curve Fitting and Calculate Relative Concentrations | Perform Noise Quality Control Calculations | Generate Graphical Output | Slide Normalization | Write Final Results | Using RPPASPACE | Setting Up Your R Environment | Organizing and Formatting Of Input Data | Creating an RPPASPACESettings Object | Example RPPASPACE Script | Outputs and Output Formats | For each successfully processed slide | One instance per slide set | Warnings and errors output files | Optional files based on settings object | Error and Message Handling | Converting Slides to Standard Slide Input Format | Display Adapter Settings on Unix | References

Background | Data File Format | Index File | Matrix Data Access | Matrix Data Type | Row Data Access